The study aimed to investigate serum vaspin and chemerin changes in response to combat physical fitness tests in military forces. In a semi-experimental design with pretest and posttest, twenty volunteers were engaged to participate in the study. Combat Physical Fitness Test consisted: (1) 25-yard(yd) sprint to J-hook, to 25-yd crawl, and 25-yd run through cones to 75-yd line; split (2) 75-yd casualty drag; split (3) ammo can run to grenade toss (75-yd); (4) ammo can run to end (75-yd); and (5) 650-yard endurance run. Pre-test and the post-test blood sample was gained. The data were analyzed by SPSS version 24. Serum chemerin did not change significantly compared to the pre-test whereas Serum vaspin was significantly higher after the test (p = 0.018). Afterward, a direct correlation was observed between serum chemerin and serum vaspin (p = 0.034, r=0.335). It is suggested that serum vaspin is responsive to short-term maximal power output activity required in the combat fitness test and positively correlates with chemerin. However, it appears that 3 to 4 minutes of high-intensity exercise induce comparatively moderate post-exercise serum vaspin increment, which appears to be inadequate to stimulate chemerin secretion.